The fish population was most recently studied during a 1995 summer survey. Seventeen species were collected including the following: bluegills, yellow perch, pumpkinseeds, smallmouth bass, chain pickerel, white perch, largemouth bass, redbreasted sunfish, rainbow trout, brown bullheads, golden shiners, banded killifish, yellow bullheads, black crappie, white suckers, brook trout, American eel and landlocked alewives. Northern pike, tiger muskies and brood salmon are also present.

Webster Lake offers an extremely diverse fishery with largemouth bass, smallmouth bass, chain pickerel, stocked trout and an assortment of panfish. Although recreational use is heavy, particularly during the summer months, many fish of large size are available. Jigging for yellow perch in the winter is particularly productive.

Northern pike and tiger muskies have been stocked here regularly since 1981 in order to utilize the abundant panfish population and offer the angler a chance to catch a truly large gamefish. Pike in the 20-pound range are taken every year.
Finally, Webster Lake has been stocked with brood salmon since 1992. These fish, some of which are over ten pounds in weight, offer the salmonid angler the possibility of intense battles. They will continue to be stocked whenever available, and are taken trough the ice as well as in open water.